Recombinant Protein Production Recombinant Protein Production

Y-SERVICE

Production of Recombinant Antibodies

Optimal High-Efficiency Transient Expression System
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    Development of an expression vector that is expressed and produced well in HEK293 cells (Ymax®-tEXPRESS system)

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    High-expression antibody protein production with the Expi-CHO system

Recombinant Antibody Production
  • Production is possible through synthesis using just the antibody sequence
  • Productivity, purity, and physical properties confirmed by expression testing within 10 days / 14 days for Expi-CHO
  • Customized production in small (mg) to large (g) scales (up to 42 L using 6 Multitron units)
  • If less than 1 L of proteins is required, it can be done within 2 weeks
  • Can change the isotype of the antibody (human, mouse)

* The development schedule may vary depending on the specifics of the project.

Recombinant Antibody Production Process
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    DNA

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    Cloning

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    Transient
    Expression

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    Purification
    Quality Control

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    Delivery

DNA
  • Antibody DNA (HC/LC)
    DNA sequence-word file
    Plasmid : 1㎍
    Leader Selection : Y-bio, Other
  • Only needs to obtain the gene sequence
    A.A sequence-word file
    Gene synthesis-Outsourcing (4~6 weeks)
    Codon-optimization selection : HEK, CHO
    Leader Selection : Y-bio, Other
Cloning
Using the User’s Expression Vector
AntibodyHC : 500 / 1L, LC : 500 / 1L (Production scale)
DNA PreparationMidi-prep, Purity >95%, RNA contamination check
HEK293F Expi-CHO
Scale(ML) HC(㎍) LC(㎍) HC(㎍) LC(㎍)
40 20 20 25 25
1000 500 500 625 625
Using Y-Bio’s Expression Vector
N293F Expression vector

Antibody

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Transient
Expression

Expi-CHO Production 4WEEKS

  • 5 DAYS Gene Cloning

    Y-Biologics Expression
    system

  • 14 DAYS Transfection

    Expi-CHO transient
    expression

  • 2 DAYS Purification

    Affinity chromatography
    (Ni-NTA, ProteinA)

  • 1 DAY Quality Control

    FPLC & HPLC

  • 1 DAY Delivery

    Delivery quick service

Scale Service Cell lines Volume Timeline Deliverables
Small Scale
  • Gene cloning
  • Transient protein expression
  • Purification
Expi-CHO 35~500 ml 3 weeks Expression data,
Purified protein
* Large Scale
  • Gene cloning
  • Transient protein expression
  • Purification
Expi-CHO 1L~42L 8~12 weeks Expression report,
Purified protein

* In case of large-scale (1 L or more) production, you can purchase the culture medium at an affordable price if you do wish to skip the purification step

HEK293F Production 3WEEKS

  • 5 DAYS Gene Cloning

    Y-Biologics Expression
    system

  • 6 DAYS Transfection

    HEK293F transient
    expression

  • 2 DAYS Purification

    Affinity chromatography
    (Ni-NTA, ProteinA)

  • 1 DAY Quality Control

    FPLC & HPLC

  • 1 DAY Delivery

    Delivery quick service

Scale Service Cell lines Volume Timeline Deliverables
Small Scale
  • Gene cloning
  • Transient protein expression
  • Purification
HEK293F 40~500 ml 2~3 weeks Expression data,
Purified protein
* Large Scale
  • Gene cloning
  • Transient protein expression
  • Purification
HEK293F 1L~42L 4~8 weeks Expression report,
Purified protein

* In case of large-scale (1 L or more) production, you can purchase the culture medium at an affordable price if you do wish to skip the purification step.

Purification
Quality Control
Primary purification (Protein A)
Good quality
Multiful Production
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Secondary purification & QC
2nd Purification & QC
AKTA pure (2ea)
uHPLC system (2ea)
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Delivery
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Delivery on the date specified by the customer

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Quick delivery service